Specific pcr products of pcr reaction mixture without any purification. Instaclone pcr cloning kit has been combined with clonejdt transformaid bacterial transformation kit a set of solutions for preparation of chemically competent e. Hi, i wanted to ask, how can i release an inserted dna into pjet1. Linearized positive selection cloning vector with a lethal insert that allows for efficient recovery of bluntended pcr.
Dna polymerase, thermo scientific dreamtaq dna polymerase or other thermostable dna polymerases. Left plate serves as the control, with vector backbone only, right plate contains pcr insert. Baculovirus was generated using the bactobac cloning system life technologies inc. Pcr cloning with lowno background a 500 bp pcr product incubated with the linearized vector in a 3. Easily share your publications and get them in front of issuus. Thermo scientific clonejet pcr cloning kit, also available with dh10b cells, is an advanced positive selection system for highefficiency cloning of pcr products generated with any thermostable dna polymerase. The clonejet pcr cloning kit is compatible with all pcr buffers supplied by thermo scientific.
Clonejet pcr cloning kit thermo fisher scientific us. Here, we assess evidence for ecological specialization and predictability of finescale population. Ligation into the positive selection vector takes only five minutes, yielding more than 99% recombinant clones. Thermo scientific clonejet pcr cloning kit, also available with dh10b cells k123120, is an advanced positive selection system for highefficiency cloning of pcr products generated with any thermostable dna polymerase. See attached the thermo scientific clonejet pcr cloning kit. Thermo scientific clonejet pcr cloning kit is an advanced positive selection system for highefficiency cloning of pcr products generated with any thermostable dna polymerase. Molecular cloning produces identical, recombinant dna molecules for downstream applications, such clonehet gene expression, protein purification, and mutational analysis. Any other blunt or stickyend dna fragment can be cloned. The thermo scientific clonejet pcr cloning kit is a versatile, advanced positive selection system for highefficiency cloning of pcr products. The neb pcr cloning kit contains a sufficient supply of materials to perform 20 x 10. For descriptions of symbols on product labels or product documents, go to. All components of the kit were function tested in control experiment as described in the manual. The clonejet pcr cloning kit is an advanced positive selection system for the highest efficiency cloning of pcr products generated with. Pcr master mix is a 2x concentrated solution of taq.
Refer to the competent cell manual for transformation protocol. It is ideal for phosphorylated or nonphosphorylated dna fragments. Specific pcr products of instaclone pcr cloning kit has been combined with the transformaid bacterial transformation kit a set of solutions for preparation of chemically competent e. Clonejet pcr cloning kit k k 20 40 t7 promoter clonejet and transformaid are. The resulting pcr product was cloned into clonejet pcr cloning kit thermofisher scientific followed by sequencing of several clones. Multiple cloning site mcs mapping, screening and excision of the cloned insert 422328 insertion site blunt dna ends for ligation with insert 3772 primer binding sites.
Molecular cloning using polymerase chain reaction, an educational. The clonejet pcr cloning kit is compatible with all pcr buffers supplied by fermentas. Gelanalyze the pcr product for specificity and yield before cloning. Transgenic expression of the antiparasitic factor tep1 in. Thermo scientific clonejet pcr cloning kit is a versatile positive selection system for highefficiency cloning of pcr products generated with any thermostable. According to our protocol, ligation and preparation of competent cells is performed in parallel. Thermo scientific clonejet pcr cloning kit, also available with dh10b cells k123120, is an advanced positive selection system for highefficiency cloning of. Primers are also provided, allowing screening for inserts by colony pcr andor sequencing. Thermo scientific magjet plant genomic dna kit is qualified by. The clonejet pcr cloning kit is an advanced positive selection system. A 2 l aliquot of the ligation mixture was used to transform 50 l of. There are several pcr cloning kits available some of which are shown in table 2.
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